Molecular characterization of porcine Siglec-10 and analysis of its expression in blood and tissues

Dev Comp Immunol. 2015 Jan;48(1):116-23. doi: 10.1016/j.dci.2014.09.011. Epub 2014 Oct 2.

Abstract

Siglecs are sialic acid binding Ig-like proteins involved in the control of leukocyte responses. In this study we describe the characterization of a porcine orthologue of Siglec-10. A cDNA clone was obtained from a porcine library which encodes a protein with sequence homology to human Siglec-10. This cDNA codes for a type I transmembrane protein containing four Ig-like domains, a transmembrane region, and a cytoplasmic tail with three tyrosine-based motifs, including a membrane-proximal Grb2-binding motif, and two ITIM motifs. When expressed on transfected cells, porcine Siglec-10 was able to bind red blood cells in a sialic acid-dependent manner. Monoclonal antibodies were developed against this protein and used to examine its cell and tissue distribution in the pig. Siglec-10 was found to be expressed on blood B cells and B cell areas of the spleen and lymph nodes. A weak expression was also detected on monocytes.

Keywords: B cells; Monoclonal antibody; Siglec-10; Swine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal / immunology
  • B-Lymphocytes / metabolism*
  • Base Sequence
  • CHO Cells
  • Cell Line
  • Cricetulus
  • Erythrocytes / metabolism*
  • Lymph Nodes / metabolism
  • Mice
  • Monocytes / metabolism
  • Protein Binding
  • Protein Structure, Tertiary
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Sialic Acid Binding Immunoglobulin-like Lectins / biosynthesis
  • Sialic Acid Binding Immunoglobulin-like Lectins / genetics
  • Sialic Acid Binding Immunoglobulin-like Lectins / immunology*
  • Sialic Acid Binding Immunoglobulin-like Lectins / metabolism*
  • Spleen / metabolism
  • Swine / genetics

Substances

  • Antibodies, Monoclonal
  • Sialic Acid Binding Immunoglobulin-like Lectins