Identification of a pentatricopeptide repeat RNA editing factor in Physcomitrella patens chloroplasts

Mizuho Ichinose; Masato Uchida; Mamoru Sugita

doi:10.1016/j.febslet.2014.09.031

Identification of a pentatricopeptide repeat RNA editing factor in Physcomitrella patens chloroplasts

FEBS Lett. 2014 Nov 3;588(21):4060-4. doi: 10.1016/j.febslet.2014.09.031. Epub 2014 Sep 30.

Authors

Mizuho Ichinose¹, Masato Uchida¹, Mamoru Sugita²

Affiliations

¹ Center for Gene Research, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan.
² Center for Gene Research, Nagoya University, Chikusa-ku, Nagoya 464-8602, Japan. Electronic address: sugita@gene.nagoya-u.ac.jp.

PMID: 25277299
DOI: 10.1016/j.febslet.2014.09.031

Abstract

The moss Physcomitrella patens has two RNA editing sites in the chloroplasts. Here we identified a novel DYW-subclass pentatricopeptide repeat (PPR) protein, PpPPR_45, as a chloroplast RNA editing factor in P. patens. Knockdown of the PpPPR_45 gene reduced the extent of RNA editing at the chloroplast rps14-C2 site, whereas over-expression of PpPPR_45 increased the levels of RNA editing at both the rps14-C2 site and its neighboring C site. This indicates that the expression level of PpPPR_45 affects the extent of RNA editing at the two neighboring sites.

Keywords: Chloroplast; DYW domain; Pentatricopeptide repeat protein; Physcomitrella patens; RNA editing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Bryopsida / genetics
Bryopsida / metabolism*
Chloroplasts / metabolism*
Gene Knockdown Techniques
Molecular Sequence Data
Photosynthesis
Plant Proteins / chemistry*
Plant Proteins / metabolism*
Protein Transport
RNA Editing*
RNA, Messenger / genetics
RNA, Messenger / metabolism
Repetitive Sequences, Amino Acid*

Substances

Plant Proteins
RNA, Messenger