[Effect of chitooligosaccharides with different degrees of acetylation on the activity of wheat pathogen-inducible anionic peroxidase]

Prikl Biokhim Mikrobiol. 2014 Jan-Feb;50(1):95-100. doi: 10.7868/s0555109913060123.
[Article in Russian]

Abstract

The effect of chitooligosaccharides (CHOSs) with a molecular weight of 5-10 kDa and a degree of acetylation (DA) of 65 and 13% at a concentration of 1.0 mg/L on the expression of the TC151917 gene, which encodes wheat anionic peroxidase, and the activity of "anionic" isoperoxidases in common wheat plants infected with Septoria nodorum Berk.--the causative agent of septoriosis. Treatment with CHOSs with a 65% DA and infection promoted the transcription of the anionic peroxidase gene and increased the enzymatic activity of the anionic peroxidase with an isoelectric point of 3.5 in soluble and ion-bound to cell walls protein fractions. Chitooligosaccharides with a 13% DA change these parameters to a lesser extent. These data suggest the importance of the degree of acetylation of CHOSs in the development of immune responses of wheat with the involvement of peroxidases.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation
  • Chitin / analogs & derivatives
  • Chitin / pharmacology*
  • Gene Expression Regulation, Plant / drug effects*
  • Isoelectric Point
  • Molecular Weight
  • Peroxidases / biosynthesis
  • Peroxidases / genetics*
  • Peroxidases / immunology
  • Plant Diseases / genetics*
  • Plant Diseases / immunology
  • Plant Diseases / microbiology
  • Plant Immunity / drug effects
  • Plant Immunity / genetics
  • Plant Proteins / biosynthesis
  • Plant Proteins / genetics*
  • Plant Proteins / immunology
  • Saccharomycetales / pathogenicity
  • Saccharomycetales / physiology
  • Transcription, Genetic / drug effects
  • Triticum / genetics*
  • Triticum / immunology
  • Triticum / microbiology

Substances

  • Plant Proteins
  • Chitin
  • Peroxidases
  • anionic peroxidase