Ruling out false-positive urinary Legionella pneumophila serogroup 1 and Streptococcus pneumoniae antigen test results by heating urine

C Pontoizeau; L Dangers; V Jarlier; C E Luyt; E Guiller; M H Fievet; M Lecsö-Bornet; A Aubry; F Brossier

doi:10.1128/JCM.01916-14

Ruling out false-positive urinary Legionella pneumophila serogroup 1 and Streptococcus pneumoniae antigen test results by heating urine

J Clin Microbiol. 2014 Dec;52(12):4347-9. doi: 10.1128/JCM.01916-14. Epub 2014 Sep 24.

Authors

C Pontoizeau¹, L Dangers², V Jarlier³, C E Luyt², E Guiller⁴, M H Fievet⁴, M Lecsö-Bornet⁵, A Aubry³, F Brossier⁶

Affiliations

¹ AP-HP, Hôpital Pitié-Salpêtrière, Bactériologie-Hygiène, Paris, France.
² AP-HP, Hôpital Pitié-Salpêtrière, Service de Réanimation Médicale, Paris, France.
³ PRES Sorbonne Universités, UPMC Universite Paris 06, INSERM, U1135, Centre for Immunology and Microbial Infections, Team 13, Paris, France AP-HP, Hôpital Pitié-Salpêtrière, Bactériologie-Hygiène, Paris, France.
⁴ AP-HP, Hôpital Pitié-Salpêtrière, Pharmacie, Paris, France.
⁵ AP-HP, Hôpital Pitié-Salpêtrière, Bactériologie-Hygiène, Paris, France Department of Microbiology, Paris-Descartes University, Paris, France.
⁶ PRES Sorbonne Universités, UPMC Universite Paris 06, INSERM, U1135, Centre for Immunology and Microbial Infections, Team 13, Paris, France AP-HP, Hôpital Pitié-Salpêtrière, Bactériologie-Hygiène, Paris, France florence.brossier@psl.aphp.fr.

Abstract

We report here false-positive urinary Legionella pneumophila serogroup 1 and Streptococcus pneumoniae antigen test results due to rabbit antilymphocyte serum treatment and provide a simple and fast solution to rule them out by heating urine.

Publication types

Case Reports

MeSH terms

Adolescent
Animals
Antigens, Bacterial / urine*
False Positive Reactions
Hot Temperature*
Humans
Legionella pneumophila / isolation & purification*
Legionellosis / diagnosis*
Male
Pneumococcal Infections / diagnosis*
Specimen Handling / methods*
Streptococcus pneumoniae / isolation & purification*
Urine / chemistry
Urine / microbiology

Substances

Antigens, Bacterial