A method has been optimized and validated for the determination of fomesafen in soils using solid-liquid extraction with low-temperature partitioning (SLE/LTP) and analysis by liquid chromatography with a high-efficiency diode array detector (HPLC/DAD). The method was used to evaluate the persistence and mobility of this herbicide in different soils cultivated with bean plants. Recovery values were ≥98.9 %, with variations in the repeatability coefficients of ≤15 %, and a detection limit of 7.3 μg kg(-1). Half-life values of fomesafen were between 60 and 71 days in soil cultivated using a no-till system and 99 and 114 days in soil cultivated using a conventional tillage system. The mobility of fomesafen was moderate and mainly influenced by the organic matter content, pH, and soil type. In Red-Yellow Argisol, which has a higher content of organic matter, the leaching of fomesafen was less pronounced. In Red-Yellow Latosol, which has smaller amounts of organic matter and high pH, the leaching of fomesafen was more pronounced.