Some findings in transgalactosylations employing modified donor substrates

Lars Kröger; Joachim Thiem

doi:10.1016/j.carres.2014.08.007

Some findings in transgalactosylations employing modified donor substrates

Carbohydr Res. 2014 Oct 29:398:8-12. doi: 10.1016/j.carres.2014.08.007. Epub 2014 Aug 18.

Authors

Lars Kröger¹, Joachim Thiem²

Affiliations

¹ University of Hamburg, Faculty of Science, Department of Chemistry, Martin-Luther-King-Platz 6, D-20146 Hamburg, Germany.
² University of Hamburg, Faculty of Science, Department of Chemistry, Martin-Luther-King-Platz 6, D-20146 Hamburg, Germany. Electronic address: thiem@chemie.uni-hamburg.de.

PMID: 25238124
DOI: 10.1016/j.carres.2014.08.007

Abstract

The scope of transgalactosylation with β-galactosidase (bovine testis) was studied employing a series of modified donor substrates based on p-nitrophenyl β-D-galactopyranoside and as uniform acceptor allyl 2-N-acetamido-2-deoxy-α-D-galactopyranoside. Structurally diverse donor molecules were recognized by the enzyme and led to novel disaccharide components, yet an excessive structural distortion was not accepted.

Keywords: Modified T-antigen components; Modified p-nitrophenyl glycopyranosides; Transgalactosylation; β-Galactosidase (bovine testis).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Galactose / metabolism*
Glycosylation
Male
Testis / enzymology
beta-Galactosidase / metabolism

Substances

beta-Galactosidase
Galactose