Potential of Monoraphidium minutum for carbon sequestration and lipid production in response to varying growth mode

Bioresour Technol. 2014 Nov:172:32-40. doi: 10.1016/j.biortech.2014.08.070. Epub 2014 Aug 23.

Abstract

Mixotrophic growth at flask level and, autotrophic-mixotrophic and autotrophic growth in photobioreactor by utilizing CO2/air/flue gas were checked for the isolated strain of Monoraphidium minutum from polluted habitat. Our study confirmed that it is a saturated fatty acid rich (30.92-68.94%) microalga with lower degree of unsaturation oil quality (42.06-103.99) making it potential biodiesel producing candidate. It showed encouraging biomass productivity (80.3-303.8mgl(-1)day(-1)) with higher total lipid (22.80-46.54%) under optimum glucose, fructose, microalgal biodiesel waste residue and sodium acetate fed mixotrophic conditions. The pH control by intermittent CO2, continuous illumination with 30% flue gas, and utilization of biodiesel glycerin were effective schemes to ameliorate either biomass productivity or % lipids or both of these parameters at photobioreactor scale (7.5L working volume). The modulation of environmental variables (pH control, CO2 and organic substrates concentration) could augment % saturated fatty acids, such as C16:0.

Keywords: Biodiesel; Flue gas; Intermittent CO(2) supply; Mixotrophic; Monoraphidium minutum.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autotrophic Processes
  • Biofuels / microbiology
  • Biomass
  • Carbon / analysis
  • Carbon Sequestration*
  • Fatty Acids / analysis
  • Freeze Drying
  • Lipids / biosynthesis*
  • Microalgae / growth & development*
  • Microalgae / metabolism*
  • Microalgae / ultrastructure
  • Nitrogen / analysis
  • Organic Chemicals / analysis
  • Photobioreactors / microbiology
  • Solubility

Substances

  • Biofuels
  • Fatty Acids
  • Lipids
  • Organic Chemicals
  • Carbon
  • Nitrogen