The new sulfated O-specific polysaccharide from marine bacterium Cobetia pacifica KMM 3878, containing 3,4-O-[(S)-1-carboxyethylidene]-D-galactose and 2,3-O-disulfate-D-galactose

Abstract

The O-specific polysaccharide was isolated from the lipopolysaccharide of Cobetia pacifica KMM 3878 and studied by chemical methods along with (1)H and (13)C NMR spectroscopy, including, 1D TOCSY and 2D (1)H, (1)H COSY, (1)H, (13)C HSQC, (1)H, (1)H ROESY, (1)H, (13)C HMBC and (1)H, (13)C H2BC experiments. The following new structure of the sulfated O-polysaccharide from C. pacifica KMM 3878 containing 3,4-O-[(S)-1-carboxyethylidene]-D-galactose and 2,3-O-disulfate-D-galactose was established: →4)-β-D-Gal2,3R-(1→6)-β-D-Gal3,4(S-Pyr)-(1→6)-β-D-Gal-(1→ Where R is -SO3H.

Keywords: 2,3-O-disulfate-d-galactose; 3,4-O-[(S)-1-carboxyethylidene]-d-galactose; Cobetia pacifica; Marine bacterium; O-specific polysaccharide; Sulfate.