Production of olive oil enriched with medium chain fatty acids catalysed by commercial immobilised lipases

P A Nunes; P Pires-Cabral; S Ferreira-Dias

doi:10.1016/j.foodchem.2011.01.071

Production of olive oil enriched with medium chain fatty acids catalysed by commercial immobilised lipases

Food Chem. 2011 Aug 1;127(3):993-8. doi: 10.1016/j.foodchem.2011.01.071. Epub 2011 Jan 25.

Authors

P A Nunes¹, P Pires-Cabral¹, S Ferreira-Dias²

Affiliations

¹ ISE, Universidade do Algarve, Campus da Penha, 8005-139 Faro, Portugal; Instituto Superior de Agronomia, CEER-Biosystems Engineering, Technical University of Lisbon, Tapada da Ajuda, 1349-017 Lisbon, Portugal.
² Instituto Superior de Agronomia, CEER-Biosystems Engineering, Technical University of Lisbon, Tapada da Ajuda, 1349-017 Lisbon, Portugal. Electronic address: suzanafdias@mail.telepac.pt.

PMID: 25214088
DOI: 10.1016/j.foodchem.2011.01.071

Abstract

Structured triacylglycerols, containing medium chain fatty acids, were produced by acidolysis of virgin olive oil with caprylic or capric acid, at a molar ratio of olive oil:fatty acid of 1:2, at 45°C for 24h, in solvent-free media or in n-hexane, catalysed by Thermomyces lanuginosa (Lipozyme TL IM), Rhizomucor miehei (Lipozyme RM IM) and Candida antarctica (Novozym 435) immobilised lipases. Incorporations were always greater for capric than for caprylic acid. For both acids, higher incorporations were always attained in solvent-free media: the highest caprylic acid incorporations were obtained with Novozym 435 (25.5mol%) and Lipozyme RM IM (25.7mol%), while similar capric acid incorporations were obtained with all biocatalysts (27.1-30.4mol%). After 10 repeated uses of Lipozyme RM IM, the same incorporation level of capric acid was obtained at the end of each 23h batch. However, with caprylic acid, a first-order deactivation was observed (half-life time, t1/2=299h). During acidolysis with both acids, Novozym 435 (t1/2=217h, for caprylic, t1/2=225h, for capric acid) and Lipozyme TL IM (t1/2=50.4h, for caprylic; t1/2=47.2h, for capric acid) presented first-order deactivation. All biocatalysts presented 1,3-regioselectivity.

Keywords: Acidolysis; Capric acid; Caprylic acid; Commercial immobilised lipases; Structured triacylglycerols; Virgin olive oil.