Determination of 3,4-dihydroxyphenylglycol, hydroxytyrosol and tyrosol purified from olive oil by-products with HPLC in animal plasma and tissues

Guillermo Rodríguez-Gutiérrez; Sharon Wood; Juan Fernández-Bolaños Guzmán; Garry G Duthie; Baukje de Roos

doi:10.1016/j.foodchem.2010.12.044

Determination of 3,4-dihydroxyphenylglycol, hydroxytyrosol and tyrosol purified from olive oil by-products with HPLC in animal plasma and tissues

Food Chem. 2011 Jun 15;126(4):1948-52. doi: 10.1016/j.foodchem.2010.12.044. Epub 2010 Dec 10.

Authors

Guillermo Rodríguez-Gutiérrez¹, Sharon Wood², Juan Fernández-Bolaños Guzmán³, Garry G Duthie², Baukje de Roos²

Affiliations

¹ University of Aberdeen, Rowett Institute of Nutrition & Health, Aberdeen, UK. Electronic address: guirogu@cica.es.
² University of Aberdeen, Rowett Institute of Nutrition & Health, Aberdeen, UK.
³ Instituto de la Grasa (CSIC), Sevilla, Spain.

PMID: 25213981
DOI: 10.1016/j.foodchem.2010.12.044

Abstract

A simple, fast and reliable method to quantify, simultaneously, 3,4-dihydroxyphenylglycol (DHPG), hydroxytyrosol (HT) and tyrosol (Ty) extracted and purified successfully from olive oil by-product, called alperujo, in animal plasma and tissues samples has been developed using a high-performance liquid chromatographic (HPLC) method with UV-Vis detection. Extraction of compounds is based on solid-phase extraction for plasma and homogenisation with zirconia beads and centrifugation for tissues. Calibration curves were linear for all three phenols at a relatively low concentration range (0.05-50μg/mL). This method has acceptable accuracy (91-95% in plasma and 63-100% in tissues), precision (1.11-8.26% intra-day and 0.32-9.5% inter-day) and sensitivity for detecting low concentrations of these phenols in small plasma volumes and several animal tissues such as liver, heart, kidney, muscle, testes, white adipose tissue (WAT) and brain.

Keywords: 3,4-Dihydroxyphenylglycol; Alperujo; Animal plasma; Animal tissues; Anti-oxidant; HPLC; Hydroxytyrosol; Olive oil; Tyrosol.