Microsatellite markers for Leucobryum boninense (Leucobryaceae), endemic to the Bonin Islands, Japan

Emiko Oguri; Tomio Yamaguchi; Tadashi Kajita; Noriaki Murakami

doi:10.3732/apps.1200399

Microsatellite markers for Leucobryum boninense (Leucobryaceae), endemic to the Bonin Islands, Japan

Appl Plant Sci. 2013 Apr 23;1(5):apps.1200399. doi: 10.3732/apps.1200399. eCollection 2013 May.

Authors

Emiko Oguri¹, Tomio Yamaguchi², Tadashi Kajita³, Noriaki Murakami¹

Affiliations

¹ Makino Herbarium, Graduate School of Science and Engineering, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji, Tokyo, 192-0397, Japan.
² Department of Biological Science, Graduate School of Science, Hiroshima University, 1-3-1 Kagamiyama, Higashi-hiroshima, Hiroshima, 739-8526, Japan.
³ Department of Biology, Graduate School of Science, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba, 263-8522, Japan.

Abstract

Premise of the study: Microsatellite primers were developed for Leucobryum boninense, endemic to the Bonin Islands, Japan, to investigate its level of genetic diversity and population genetic structure. •

Methods and results: Using next-generation sequencing, 21 primer sets were developed, among which nine loci were polymorphic in the populations of the Bonin Islands. Among these polymorphic loci, the number of alleles per locus ranged from two to 10 (mean = 3.444) and the expected heterozygosity ranged from 0.066 to 0.801 (mean = 0.338). •

Conclusions: These results indicate the utility of the nine microsatellite markers that we developed for population genetic studies of L. boninense.

Keywords: Bonin (Ogasawara) Islands; Leucobryaceae; Leucobryum boninense; Musci; microsatellite; next-generation sequencing.