Isolation and characterization of 27 microsatellite markers for the endemic species Diplarche multiflora (Ericaceae)

Lin Zhang; Zhi-Rong Zhang; Lian-Ming Gao

doi:10.3732/apps.1200235

Isolation and characterization of 27 microsatellite markers for the endemic species Diplarche multiflora (Ericaceae)

Appl Plant Sci. 2013 Jan 2;1(1):apps.1200235. doi: 10.3732/apps.1200235. eCollection 2013 Jan.

Authors

Lin Zhang¹, Zhi-Rong Zhang², Lian-Ming Gao³

Affiliations

¹ Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, People's Republic of China ; Graduate School of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
² Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, People's Republic of China.
³ Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, People's Republic of China.

Abstract

Premise of the study: Microsatellite markers from the genome of Diplarche multiflora were developed and characterized to investigate its genetic diversity and population structure. •

Methods and results: Twenty-seven microsatellite loci were isolated from the genome of D. multiflora using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol. Of these markers, 17 were polymorphic, and the number of alleles for the polymorphic microsatellite markers ranged from two to four, with an average of 2.2 per allele. The observed and expected heterozygosities varied from 0.0000 to 1.0000 and from 0.0000 to 0.7826, respectively. •

Conclusions: These polymorphic microsatellite markers will be useful for population genetic studies and for assessing the genetic diversity of this alpine species.

Keywords: Diplarche multiflora; Ericaceae; FIASCO; microsatellite markers; polymorphism.