Genome sequence of the acid-tolerant Burkholderia sp. strain WSM2232 from Karijini National Park, Australia

Robert Walker; Elizabeth Watkin; Rui Tian; Lambert Bräu; Graham O'Hara; Lynne Goodwin; James Han; Tatiparthi Reddy; Marcel Huntemann; Amrita Pati; Tanja Woyke; Konstantinos Mavromatis; Victor Markowitz; Natalia Ivanova; Nikos Kyrpides; Wayne Reeve

doi:10.4056/sigs.5018795

Genome sequence of the acid-tolerant Burkholderia sp. strain WSM2232 from Karijini National Park, Australia

Stand Genomic Sci. 2013 Nov 15;9(3):1168-80. doi: 10.4056/sigs.5018795. eCollection 2014 Jun 15.

Authors

Affiliations

¹ School of Biomedical Sciences, Faculty of Health Sciences, Curtin University, Western Australia, Australia.
² Centre for Rhizobium Studies, School of Veterinary and Life Sciences, Murdoch University, Western Australia, Australia.
³ School of Life and Environmental Sciences, Deakin University, Victoria, Australia.
⁴ Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
⁵ DOE Joint Genome Institute, Walnut Creek, California, USA.
⁶ Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, California, USA.

Abstract

Burkholderia sp. strain WSM2232 is an aerobic, motile, Gram-negative, non-spore-forming acid-tolerant rod that was trapped in 2001 from acidic soil collected from Karijini National Park (Australia) using Gastrolobium capitatum as a host. WSM2232 was effective in nitrogen fixation with G. capitatum but subsequently lost symbiotic competence during long-term storage. Here we describe the features of Burkholderia sp. strain WSM2232, together with genome sequence information and its annotation. The 7,208,311 bp standard-draft genome is arranged into 72 scaffolds of 72 contigs containing 6,322 protein-coding genes and 61 RNA-only encoding genes. The loss of symbiotic capability can now be attributed to the loss of nodulation and nitrogen fixation genes from the genome. This rhizobial genome is one of 100 sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project.

Keywords: Betaproteobacteria; nitrogen fixation; rhizobia; root-nodule bacteria.