Genome sequence of the acid-tolerant Burkholderia sp. strain WSM2230 from Karijini National Park, Australia

Robert Walker; Elizabeth Watkin; Rui Tian; Lambert Bräu; Graham O'Hara; Lynne Goodwin; James Han; Elizabeth Lobos; Marcel Huntemann; Amrita Pati; Tanja Woyke; Konstantinos Mavromatis; Victor Markowitz; Natalia Ivanova; Nikos Kyrpides; Wayne Reeve

doi:10.4056/sigs.5008793

Genome sequence of the acid-tolerant Burkholderia sp. strain WSM2230 from Karijini National Park, Australia

Stand Genomic Sci. 2013 Dec 31;9(3):551-61. doi: 10.4056/sigs.5008793. eCollection 2014 Jun 15.

Authors

Affiliations

¹ School of Biomedical Sciences, Faculty of Health Sciences, Curtin University, Western Australia, Australia.
² Centre for Rhizobium Studies, School of Veterinary and Life Sciences, Murdoch University, Western Australia, Australia.
³ School of Life and Environmental Sciences, Deakin University, Victoria, Australia.
⁴ Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico, USA.
⁵ DOE Joint Genome Institute, Walnut Creek, California, USA.
⁶ Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, California, USA.

Abstract

Burkholderia sp. strain WSM2230 is an aerobic, motile, Gram-negative, non-spore-forming acid-tolerant rod isolated from acidic soil collected in 2001 from Karijini National Park, Western Australia, using Kennedia coccinea (Coral Vine) as a host. WSM2230 was initially effective in nitrogen-fixation with K. coccinea, but subsequently lost symbiotic competence. Here we describe the features of Burkholderia sp. strain WSM2230, together with genome sequence information and its annotation. The 6,309,801 bp high-quality-draft genome is arranged into 33 scaffolds of 33 contigs containing 5,590 protein-coding genes and 63 RNA-only encoding genes. The genome sequence of WSM2230 failed to identify nodulation genes and provides an explanation for the observed failure of the laboratory grown strain to nodulate. The genome of this strain is one of 100 sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project.

Keywords: Betaproteobacteria; nitrogen fixation; rhizobia; root-nodule bacteria.