Phylogenetic and transcription analysis of chrysanthemum WRKY transcription factors

Aiping Song; Peiling Li; Jiafu Jiang; Sumei Chen; Huiyun Li; Jun Zeng; Yafeng Shao; Lu Zhu; Zhaohe Zhang; Fadi Chen

doi:10.3390/ijms150814442

Phylogenetic and transcription analysis of chrysanthemum WRKY transcription factors

Int J Mol Sci. 2014 Aug 19;15(8):14442-55. doi: 10.3390/ijms150814442.

Authors

Aiping Song¹, Peiling Li², Jiafu Jiang³, Sumei Chen⁴, Huiyun Li⁵, Jun Zeng⁶, Yafeng Shao⁷, Lu Zhu⁸, Zhaohe Zhang⁹, Fadi Chen¹⁰

Affiliations

¹ College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. aiping_song@aliyun.com.
² College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 2011204026@njau.edu.cn.
³ College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. jiangjiafu@njau.edu.cn.
⁴ College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. chensm@njau.edu.cn.
⁵ College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 2010204024@njau.edu.cn.
⁶ College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 2010104110@njau.edu.cn.
⁷ College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 2010104109@njau.edu.cn.
⁸ College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 2011104109@njau.edu.cn.
⁹ College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 2012104099@njau.edu.cn.
¹⁰ College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. chenfd@njau.edu.cn.

Abstract

WRKY transcription factors are known to function in a number of plant processes. Here we have characterized 15 WRKY family genes of the important ornamental species chrysanthemum (Chrysanthemum morifolium). A total of 15 distinct sequences were isolated; initially internal fragments were amplified based on transcriptomic sequence, and then the full length cDNAs were obtained using RACE (rapid amplification of cDNA ends) PCR. The transcription of these 15 genes in response to a variety of phytohormone treatments and both biotic and abiotic stresses was characterized. Some of the genes behaved as would be predicted based on their homology with Arabidopsis thaliana WRKY genes, but others showed divergent behavior.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chrysanthemum / classification*
Chrysanthemum / genetics
Chrysanthemum / metabolism*
Gene Expression Regulation, Plant
Phylogeny*
Plant Proteins / genetics
Plant Proteins / metabolism*
Transcription Factors / genetics
Transcription Factors / metabolism*

Substances

Plant Proteins
Transcription Factors