A sentinel protein assay for simultaneously quantifying cellular processes

Martin Soste; Rita Hrabakova; Stefanie Wanka; Andre Melnik; Paul Boersema; Alessio Maiolica; Timon Wernas; Marco Tognetti; Christian von Mering; Paola Picotti

doi:10.1038/nmeth.3101

A sentinel protein assay for simultaneously quantifying cellular processes

Nat Methods. 2014 Oct;11(10):1045-8. doi: 10.1038/nmeth.3101. Epub 2014 Sep 7.

Authors

Affiliations

¹ Institute of Biochemistry, Department of Biology, ETH Zurich, Zurich, Switzerland.
² 1] Institute of Biochemistry, Department of Biology, ETH Zurich, Zurich, Switzerland. [2] Laboratory of Applied Proteome Analyses, Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Libechov, Czech Republic.
³ Institute of Molecular Life Sciences and Swiss Institute of Bioinformatics, University of Zurich, Zurich, Switzerland.
⁴ Institute of Molecular Systems Biology, Department of Biology, ETH Zurich, Zurich, Switzerland.

PMID: 25194849
DOI: 10.1038/nmeth.3101

Abstract

We describe a proteomic screening approach based on the concept of sentinel proteins, biological markers whose change in abundance characterizes the activation state of a given cellular process. Our sentinel assay simultaneously probed 188 biological processes in Saccharomyces cerevisiae exposed to a set of environmental perturbations. The approach can be applied to analyze responses to large sets of uncharacterized perturbations in high throughput.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Computational Biology / methods*
Humans
Mass Spectrometry / methods
Peptides / chemistry
Phosphoproteins / chemistry
Protein Interaction Mapping
Proteomics / methods*
Reproducibility of Results
Saccharomyces cerevisiae / metabolism*
Saccharomyces cerevisiae Proteins / chemistry*
Systems Biology / methods*
Transcriptome

Substances

Peptides
Phosphoproteins
Saccharomyces cerevisiae Proteins