To pool or not to pool? Impact of the use of individual and pooled fecal samples for in vitro fermentation studies

J Microbiol Methods. 2014 Dec:107:1-7. doi: 10.1016/j.mimet.2014.08.022. Epub 2014 Sep 3.

Abstract

This study investigated the stability and the activity of the microbiota from a single and a pool of donors in the TNO in vitro model of the colon (TIM-2 system). Our findings demonstrate the suitability of the preparation of a pool of fecal sample to be used for fermentation experiments.

Keywords: Fecal inoculum preparation; Fermentation; Gut microbiota; In vitro model; Pooling.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetates / metabolism
  • Adult
  • Biodiversity
  • Butyrates / metabolism
  • Feces / microbiology*
  • Female
  • Fermentation*
  • Humans
  • In Vitro Techniques*
  • Male
  • Metagenome
  • Microbiota*
  • Middle Aged
  • Phylogeny
  • Propionates / metabolism
  • Time Factors

Substances

  • Acetates
  • Butyrates
  • Propionates