Comment on "Local impermeant anions establish the neuronal chloride concentration"

Heiko J Luhmann; Sergei Kirischuk; Werner Kilb

doi:10.1126/science.1255337

Comment on "Local impermeant anions establish the neuronal chloride concentration"

Science. 2014 Sep 5;345(6201):1130. doi: 10.1126/science.1255337.

Authors

Heiko J Luhmann¹, Sergei Kirischuk², Werner Kilb²

Affiliations

¹ Institute of Physiology, University Medical Center, University of Mainz, Duesbergweg 6, D-55128 Mainz, Germany. luhmann@uni-mainz.de.
² Institute of Physiology, University Medical Center, University of Mainz, Duesbergweg 6, D-55128 Mainz, Germany.

PMID: 25190788
DOI: 10.1126/science.1255337

Abstract

Glykys et al. (Reports, 7 February 2014, p. 670) proposed that cytoplasmic impermeant anions and polyanionic extracellular matrix glycoproteins establish the local neuronal intracellular chloride concentration, [Cl(-)]i, and thereby the polarity of γ-aminobutyric acid type A (GABAA) receptor signaling. The experimental procedures and results in this study are insufficient to support these conclusions. Contradictory results previously published by these authors and other laboratories are not referred to.

Publication types

Comment

MeSH terms

Animals
Brain / metabolism*
Chloride Channels / metabolism*
Chlorides / metabolism*
Neurons / metabolism*
Receptors, GABA-A / metabolism*

Substances

Chloride Channels
Chlorides
Receptors, GABA-A