The complete mitochondrial genome sequence of the hybrid of Siniperca kneri (♀) × Siniperca chuatsi (♂) is described in this study. The 16,488 bp long circular molecule consisted of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a control region, showed a typical vertebrate pattern. The overall base composition of the heavy strand is 28.59% A, 29.20% C, 16.25% G, and 25.96% T, with a slight AT bias of 54.55%. Except for eight tRNA and ND6 genes, all other mitochondrial genes are encoded on the heavy strand. There are 5 regions of gene overlap totaling 14 bp and 20 intergenic spacer regions totaling 91 bp. The complete mitogenome of the hybrid of S. kneri (♀) × S. chuatsi (♂) could provide an important data set for the study in mitochondrial inheritance mechanism.
Keywords: Hybrid of Siniperca kneri (♀) × Siniperca chuatsi (♂); Mitogenome; PCR.