Direct conversion of cytochrome c spectral shifts to fluorescence using photochromic FRET

Chem Commun (Camb). 2014 Oct 21;50(82):12333-6. doi: 10.1039/c4cc06146b.

Abstract

Photochromic fluorescence resonance energy transfer (pcFRET) was used to monitor the redox activity of non-fluorescent heme protein. Venus fluorescent protein was used as a donor where its emission intensity was reversibly modulated by the absorption change of Cytochrome c.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / chemistry*
  • Cytochromes c / chemistry*
  • Fluorescence Resonance Energy Transfer / methods
  • Hemeproteins / chemistry*
  • Luminescent Proteins / chemistry*
  • Oxidation-Reduction

Substances

  • Bacterial Proteins
  • Hemeproteins
  • Luminescent Proteins
  • yellow fluorescent protein, Bacteria
  • Cytochromes c