Detection of multiple mutations in a single codon of genomic DNA

Danishmalik Rafiq Sayyed; Satish Balasaheb Nimse; Keum-Soo Song; Taisun Kim

doi:10.1039/c4cc06220e

Detection of multiple mutations in a single codon of genomic DNA

Chem Commun (Camb). 2014 Oct 21;50(82):12344-7. doi: 10.1039/c4cc06220e.

Authors

Danishmalik Rafiq Sayyed¹, Satish Balasaheb Nimse, Keum-Soo Song, Taisun Kim

Affiliation

¹ Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon, 200-702, Korea. tskim@hallym.ac.kr.

PMID: 25182010
DOI: 10.1039/c4cc06220e

Abstract

The controller DNA technology allows the detection of multiple mutations in a single codon of genomic DNA. In this technology, the controller DNA is used to control the hybridization of target DNAs with the immobilized DNAs. The controller DNA technology is rapid, specific, and cost-effective for the following reasons, (i) final results in 40 min after PCR, (ii) detection and discrimination of the six mutations in a single codon, and (iii) high sensitivity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Codon / genetics*
DNA, Bacterial / genetics*
Mutation*
Mycobacterium tuberculosis / genetics
Polymerase Chain Reaction
Polymorphism, Single Nucleotide

Substances

Codon
DNA, Bacterial