Abstract
This study was conducted to evaluate the immunogenicity and protective efficacy of a DNA vaccine encoding Brucella abortus Cu,Zn superoxide dismutase (SOD) using the Toll-like receptor 2/6 agonist S-[2,3-bispalmitoyiloxy-(2R)-propyl]-R-cysteinyl-amido-monomethoxy polyethylene glycol (BPPcysMPEG) as an adjuvant. Intranasal coadministration of BPPcysMPEG with a plasmid carrying the SOD-encoding gene (pcDNA-SOD) into BALB/c mice elicited antigen-specific humoral and cellular immune responses. Humoral responses were characterized by the stimulation of IgG2a and IgG1 and by the presence of SOD-specific secretory IgA in nasal and bronchoalveolar lavage fluids. Furthermore, T-cell proliferative responses and increased production of gamma interferon were also observed upon splenocyte restimulation with recombinant SOD. Cytotoxic responses were also stimulated, as demonstrated by the lysis of RB51-SOD-infected J774.A1 macrophages by cells recovered from immunized mice. The pcDNA-SOD/BPPcysMPEG formulation induced improved protection against challenge with the virulent strain B. abortus 2308 in BALB/c mice over that provided by pcDNA-SOD, suggesting the potential of this vaccination strategy against Brucella infection.
Copyright © 2014, American Society for Microbiology. All Rights Reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adjuvants, Immunologic / administration & dosage*
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Adjuvants, Immunologic / pharmacology
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Administration, Intranasal
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Animals
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Antibodies, Bacterial / blood
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Bacterial Proteins / genetics
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Bacterial Proteins / immunology
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Bronchoalveolar Lavage Fluid / immunology
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Brucella Vaccine / administration & dosage
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Brucella Vaccine / genetics
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Brucella Vaccine / immunology*
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Brucella abortus / enzymology*
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Brucella abortus / genetics
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Brucellosis / immunology
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Brucellosis / prevention & control*
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Cell Proliferation
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Cytotoxicity Tests, Immunologic
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Disease Models, Animal
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Female
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Immunoglobulin A, Secretory / analysis
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Immunoglobulin G / blood
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Interferon-gamma / metabolism
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Macrophages / immunology
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Macrophages / microbiology
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Mice, Inbred BALB C
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Nasal Mucosa / immunology
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Polyethylene Glycols / administration & dosage*
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Polyethylene Glycols / pharmacology
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Spleen / immunology
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Superoxide Dismutase / genetics
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Superoxide Dismutase / immunology*
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T-Lymphocytes / immunology
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Toll-Like Receptor 2 / agonists
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Toll-Like Receptor 6 / agonists
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Vaccines, DNA / administration & dosage
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Vaccines, DNA / genetics
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Vaccines, DNA / immunology*
Substances
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Adjuvants, Immunologic
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Antibodies, Bacterial
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Bacterial Proteins
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Brucella Vaccine
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Immunoglobulin A, Secretory
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Immunoglobulin G
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Tlr2 protein, mouse
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Tlr6 protein, mouse
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Toll-Like Receptor 2
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Toll-Like Receptor 6
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Vaccines, DNA
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Polyethylene Glycols
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Interferon-gamma
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Superoxide Dismutase