The pyruvate-tricarboxylic acid cycle node: a focal point of virulence control in the enteric pathogen Yersinia pseudotuberculosis

J Biol Chem. 2014 Oct 24;289(43):30114-32. doi: 10.1074/jbc.M114.581348. Epub 2014 Aug 27.

Abstract

Despite our increasing knowledge of the specific pathogenicity factors in bacteria, the contribution of metabolic processes to virulence is largely unknown. Here, we elucidate a tight connection between pathogenicity and core metabolism in the enteric pathogen Yersinia pseudotuberculosis by integrated transcriptome and [(13)C]fluxome analysis of the wild type and virulence-regulator mutants. During aerobic growth on glucose, Y. pseudotuberculosis reveals an unusual flux distribution with a high level of secreted pyruvate. The absence of the transcriptional and post-transcriptional regulators RovA, CsrA, and Crp strongly perturbs the fluxes of carbon core metabolism at the level of pyruvate metabolism and the tricarboxylic acid (TCA) cycle, and these perturbations are accompanied by transcriptional changes in the corresponding enzymes. Knock-outs of regulators of this metabolic branch point and of its central enzyme, pyruvate kinase (ΔpykF), result in mutants with significantly reduced virulence in an oral mouse infection model. In summary, our work identifies the pyruvate-TCA cycle node as a focal point for controlling the host colonization and virulence of Yersinia.

Keywords: Bacteria; Bacterial Metabolism; Bacterial Pathogenesis; Gene Expression; Metabolic Flux Analysis; Pyruvate; Systems Biology; Tricarboxylic Acid Cycle (TCA Cycle) (Krebs Cycle); Yersinia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptation, Physiological / drug effects
  • Adaptation, Physiological / genetics
  • Animals
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Biomass
  • Citric Acid Cycle* / drug effects
  • Citric Acid Cycle* / genetics
  • Escherichia coli / drug effects
  • Escherichia coli / metabolism
  • Female
  • Gene Expression Regulation, Bacterial / drug effects
  • Gene Regulatory Networks
  • Glucose / pharmacology
  • Iron / pharmacology
  • Metabolic Flux Analysis
  • Mice
  • Mice, Inbred BALB C
  • Molecular Weight
  • Mutation / genetics
  • Pyruvates / metabolism*
  • Stress, Physiological / drug effects
  • Stress, Physiological / genetics
  • Transcriptome / drug effects
  • Transcriptome / genetics
  • Virulence / drug effects
  • Virulence / genetics
  • Yersinia pseudotuberculosis / genetics
  • Yersinia pseudotuberculosis / growth & development
  • Yersinia pseudotuberculosis / metabolism*
  • Yersinia pseudotuberculosis / pathogenicity*
  • Yersinia pseudotuberculosis Infections / microbiology
  • Yersinia pseudotuberculosis Infections / pathology

Substances

  • Bacterial Proteins
  • Pyruvates
  • Iron
  • Glucose