Genetic analysis and QTL detection for resistance to white tip disease in rice

PLoS One. 2014 Aug 27;9(8):e106099. doi: 10.1371/journal.pone.0106099. eCollection 2014.

Abstract

The inheritance of resistance to white tip disease (WTDR) in rice (Oryza sativa L.) was analyzed with an artificial inoculation test in a segregating population derived from the cross between Tetep, a highly resistant variety that was identified in a previous study, and a susceptible cultivar. Three resistance-associated traits, including the number of Aphelenchoides besseyi (A. besseyi) individuals in 100 grains (NA), the loss rate of panicle weight (LRPW) and the loss rate of the total grains per panicle (LRGPP) were analyzed for the detection of the quantitative trait locus (QTL) in the population after construction of a genetic map. Six QTLs distributed on chromosomes 3, 5 and 9 were mapped. qNA3 and qNA9, conferring reproduction number of A. besseyi in the panicle, accounted for 16.91% and 12.54% of the total phenotypic variance, respectively. qDRPW5a and qDRPW5b, associated with yield loss, were located at two adjacent marker intervals on chromosome 5 and explained 14.15% and 14.59% of the total phenotypic variation and possessed LOD values of 3.40 and 3.39, respectively. qDRPW9 was considered as a minor QTL and only explained 1.02% of the phenotypic variation. qLRGPP5 contributed to the loss in the number of grains and explained 10.91% of the phenotypic variation. This study provides useful information for the breeding of resistant cultivars against white tip disease in rice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chimera
  • Chromosome Mapping
  • Chromosomes, Plant / chemistry*
  • Crosses, Genetic
  • Genotype
  • Nematoda / pathogenicity
  • Nematoda / physiology
  • Oryza / genetics*
  • Oryza / immunology
  • Oryza / parasitology
  • Phenotype
  • Plant Diseases / genetics*
  • Plant Diseases / immunology
  • Plant Diseases / parasitology
  • Plant Immunity / genetics*
  • Quantitative Trait Loci*

Grants and funding

This research was financially supported by grants from the National Key Basic Research and Development Program (973 Program) of China (2013CBA01403), the Special Fund for Agro-Scientific Research in the Public Interest of China (201303021, 201003031), the Jiangsu Province Science and Technology Support Project (BE2012303) and the Jiangsu Agricultural Science and Technology Independent Innovation Fund (cx[12]1003). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.