Under greenhouse conditions, the success of five subsequent applications of A. pullulans isolate 7Ep2, in reducing symptoms of Fusarium head blight on wheat ears inoculated with the pathogen F. culmorum was analysed. The survivability of the applied antagonist on wheat ears was also assessed. Counts of cells of A. pullulans colonies on ears inoculated with isolate and 7Ep2 were 56.72% higher than in the control. The high density of this species contributed to reduced development of F. culmorum on kernels not inoculated with this pathogen. The success rate of the biological treatment in reducing symptoms of Fusarium head blight on winter wheat ears was 30.35% depending on the applied isolated, compared to the control.