Abstract
Acrolein is a reactive α,β-unsaturated aldehyde derived from lipid peroxides, which are produced in plants under a variety of stress. We investigated effects of acrolein on light-induced stomatal opening using Arabidopsis thaliana. Acrolein inhibited light-induced stomatal opening in a dose-dependent manner. Acrolein at 100 μM inhibited plasma membrane inward-rectifying potassium (Kin) channels in guard cells. Acrolein at 100 μM inhibited Kin channel KAT1 expressed in a heterologous system using Xenopus leaves oocytes. These results suggest that acrolein inhibits light-induced stomatal opening through inhibition of Kin channels in guard cells.
Keywords:
Arabidopsis thaliana; acrolein; guard cell; inward-rectifying potassium channel; light-induced stomatal opening.
MeSH terms
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Acrolein / metabolism
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Acrolein / pharmacology*
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Animals
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Arabidopsis / drug effects*
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Arabidopsis / metabolism
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Arabidopsis / radiation effects
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Arabidopsis Proteins / antagonists & inhibitors*
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism
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Dose-Response Relationship, Drug
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Gene Expression
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Kinetics
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Light
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Membrane Potentials / drug effects
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Oocytes / cytology
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Oocytes / drug effects
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Oocytes / metabolism
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Patch-Clamp Techniques
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Plant Cells / drug effects*
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Plant Cells / metabolism
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Plant Cells / radiation effects
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Plant Stomata / drug effects*
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Plant Stomata / metabolism
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Plant Stomata / radiation effects
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Potassium / metabolism*
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Potassium Channels, Inwardly Rectifying / antagonists & inhibitors*
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Potassium Channels, Inwardly Rectifying / genetics
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Potassium Channels, Inwardly Rectifying / metabolism
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Xenopus laevis
Substances
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Arabidopsis Proteins
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KAT1 protein, Arabidopsis
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Potassium Channels, Inwardly Rectifying
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Recombinant Proteins
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Acrolein
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Potassium