Total fatty acid composition of muscle lipids in some fish species (Cyprinus carpio (Işıklı Dam Lake), Tinca tinca (Işıklı Dam Lake), Scardinius erythrophthalmus (Işıklı Dam Lake), Cyprinus carpio (Karacaören Dam Lake), and Carassius carassius (Karacaören Dam Lake)) was determined by gas chromatography. Polyunsaturated fatty acids (PUFAs) of Cyprinus carpio (Işıklı Dam Lake) were found higher than PUFA of other species. Palmitic acid was the highest saturated fatty acid (SFA) in Tinca tinca (24.64%). Oleic acid was the highest monounsaturated fatty acid (MUFAs) in Cyprinus carpio (Işıklı Dam Lake) (19.25%). The most abundant polyunsaturated fatty acid in Scardinius erythrophthalmus was docosahexaenoic acid (DHA) (17.94%). Total ω3 fatty acid composition was higher than the total ω6 fatty acids of Cyprinus carpio in both dam lakes. ω3/ω6 rates in Cyprinus carpio (Işıklı Dam Lake), Tinca tinca, Scardinius erythrophthalmus, Cyprinus carpio (Karacaören), and Carassius carassius were 2.12, 1.19, 2.15, 2.87, and 2.82, respectively.