PCR-based prediction of type I β-exotoxin production in Bacillus thuringiensis strains

Diego H Sauka; Melisa P Pérez; Nanci N López; María I Onco; Marcelo F Berretta; Graciela B Benintende

doi:10.1016/j.jip.2014.08.001

PCR-based prediction of type I β-exotoxin production in Bacillus thuringiensis strains

J Invertebr Pathol. 2014 Oct:122:28-31. doi: 10.1016/j.jip.2014.08.001. Epub 2014 Aug 15.

Authors

Diego H Sauka¹, Melisa P Pérez², Nanci N López², María I Onco², Marcelo F Berretta², Graciela B Benintende²

Affiliations

¹ Instituto de Microbiología y Zoología Agrícola INTA, CC No 25, 1712 Castelar, Buenos Aires, Argentina. Electronic address: sauka.diego@inta.gob.ar.
² Instituto de Microbiología y Zoología Agrícola INTA, CC No 25, 1712 Castelar, Buenos Aires, Argentina.

PMID: 25132154
DOI: 10.1016/j.jip.2014.08.001

Abstract

Some Bacillus thuringiensis strains secrete type I β-exotoxin, which is a non-specific insecticidal and thermostable adenine nucleoside oligosaccharide. Toxicity bioassays and HPLC are traditional methods for detecting β-exotoxin. With the aim of establish a first rapid approach for prediction of type I β-exotoxin production, two PCR-based methods were successfully evaluated in B. thuringiensis strains and native isolates. In order to validate a reliable technology, results obtained by this method were correlated with that obtained from Musca domestica bioassays.

Keywords: Bacillus thuringiensis; PCR; thuE gene; β-exotoxin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine / analogs & derivatives*
Adenosine / analysis
Adenosine / biosynthesis
Bacillus thuringiensis / metabolism*
Polymerase Chain Reaction / methods*
Sugar Acids / analysis*

Substances

Sugar Acids
thuringiensin
Adenosine