Isolation and characterization of microsatellite loci for the blackstripe topminnow Fundulus notatus and their variability in two closely related species

K A Feldheim; B R Kreiser; B Schmidt; D D Duvernell; J F Schaefer

doi:10.1111/jfb.12469

Isolation and characterization of microsatellite loci for the blackstripe topminnow Fundulus notatus and their variability in two closely related species

J Fish Biol. 2014 Nov;85(5):1726-32. doi: 10.1111/jfb.12469. Epub 2014 Aug 13.

Authors

K A Feldheim¹, B R Kreiser, B Schmidt, D D Duvernell, J F Schaefer

Affiliation

¹ The Field Museum of Natural History, Pritzker Laboratory for Molecular Systematics and Evolution, 1400 S. Lake Shore Drive, Chicago, IL 60605, U.S.A.

PMID: 25123486
DOI: 10.1111/jfb.12469

Abstract

A total of 17 polymorphic microsatellite loci were isolated from the blackstripe topminnow Fundulus notatus. In a sample of 29 individuals, these loci were found to possess two to 19 alleles with expected heterozygosity values ranging from 0.212 to 0.919 and all but one of the loci conformed to Hardy-Weinberg equilibrium expectations. Many of these loci were polymorphic in the closely related species Fundulus olivaceus and Fundulus euryzonus providing a set of markers that should prove useful in future ecological and evolutionary studies of members of this species complex.

Keywords: Fundulidae; cross-species amplification; genetic marker; population genetics; primer sequence.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Alleles
Animals
Cyprinidae / genetics*
Genetic Loci
Microsatellite Repeats*
Sequence Analysis, DNA