Expression analysis of a stress-related phosphoinositide-specific phospholipase C gene in wheat (Triticum aestivum L.)

PLoS One. 2014 Aug 14;9(8):e105061. doi: 10.1371/journal.pone.0105061. eCollection 2014.

Abstract

Plant phosphoinositide-specific phospholipases C (PI-PLCs) function in several essential plant processes associated with either development or environmental stress. In this report, we examined the expression patterns of TaPLC1 under drought and high salinity stress at the transcriptional and post-transcriptional levels. TaPLC1 mRNA was expressed in all wheat organs examined. U73122 and edelfosine, the PLC inhibitor, impaired seedling growth and enhanced seedling sensitivity to drought and high salinity stress. Though TaPLC1 expression in wheat was lowest at the seedling stage, it was strongly induced under conditions of stress. When 6-day-old wheat seedlings were treated with 200 mM NaCl or 20% (w/v) PEG 6000 for 6 or 12 h, respectively, the TaPLC1 transcript level increased by 16-fold compared to the control. Western blotting showed that the TaPLC protein concentration was also maintained at a high level from 24 to 48 h during stress treatment. Together, our results indicate the possible biological functions of TaPLC1 in regulating seedling growth and the response to drought and salinity stress.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA Primers
  • Gene Expression Regulation, Enzymologic*
  • Gene Expression Regulation, Plant*
  • Genes, Plant
  • Phosphoinositide Phospholipase C / antagonists & inhibitors
  • Phosphoinositide Phospholipase C / genetics*
  • Phylogeny
  • RNA, Messenger / genetics
  • Real-Time Polymerase Chain Reaction
  • Stress, Physiological*
  • Triticum / enzymology
  • Triticum / genetics*

Substances

  • DNA Primers
  • RNA, Messenger
  • Phosphoinositide Phospholipase C

Grants and funding

This research was financially co-supported by the National Natural Science Foundation of China (Grant No. 30570993) and Hebei Natural Science Foundation Program, Hebei Province, China (No. C2008000292). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.