Diversity of selected Lupinus angustifolius L. genotypes at the phenotypic and DNA level with respect to microscopic seed coat structure and thickness

Jon Clements; Renata Galek; Bartosz Kozak; Dariusz Jan Michalczyk; Agnieszka Iwona Piotrowicz-Cieślak; Ewa Sawicka-Sienkiewicz; Stanislaw Stawiński; Dariusz Zalewski

doi:10.1371/journal.pone.0102874

Diversity of selected Lupinus angustifolius L. genotypes at the phenotypic and DNA level with respect to microscopic seed coat structure and thickness

PLoS One. 2014 Aug 13;9(8):e102874. doi: 10.1371/journal.pone.0102874. eCollection 2014.

Authors

Jon Clements¹, Renata Galek², Bartosz Kozak², Dariusz Jan Michalczyk³, Agnieszka Iwona Piotrowicz-Cieślak³, Ewa Sawicka-Sienkiewicz², Stanislaw Stawiński⁴, Dariusz Zalewski²

Affiliations

¹ Centre for Plant Genetics and Breeding, University of Western Australia, Crawley, Western Australia, Australia.
² Department of Genetics, Plant Breeding and Seed Production, Wroclaw University of Environmental and Life Sciences, Wroclaw, Lower Silesia, Poland.
³ Department of Plant Physiology and Biotechnology, University of Warmia and Mazury, Olsztyn, Warmian-Masurian, Poland.
⁴ Plant Breeding Station, "HR Smolice Sp. z o. o. Grupa IHAR" Przebedowo, Greater Poland, Poland.

Abstract

The paper investigates seed coat characteristics (as a percentage of overall seed diameter) in Lupinus angustifolius L., a potential forage crop. In the study ten L. angustifolius genotypes, including three Polish cultivars, two Australian cultivars, three mutants originated from cv. 'Emir', and one Belarusian and one Australian breeding line were evaluated. The highest seed coat percentage was recorded in cultivars 'Sonet' and 'Emir'. The lowest seed coat thickness percentage (below 20%) was noted for breeding lines 11257-19, LAG24 and cultivar 'Zeus' (17.87%, 18.91% 19.60%, respectively). Despite having low seed weight, the Australian line no. 11257-19 was characterized by a desirable proportion of seed coat to the weight of seeds. In general, estimation of the correlation coefficient indicated a tendency that larger seeds had thinner coats. Scanning Electron Microscopy images showed low variation of seed coat sculpture and the top of seeds covered with a cuticle. Most of the studied genotypes were characterized by a cristatepapillate seed coat surface, formed by elongated polygonal cells. Only breeding line no. 11267-19 had a different shape of the cells building the surface layer of the coat. In order to illustrate genetic diversity among the genotypes tested, 24 ISSR primers were used. They generated a total of 161 polymorphic amplification products in 10 evaluated narrow-leaved lupin genotypes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cluster Analysis
Genetic Association Studies
Genetic Variation
Genotype
Lupinus / anatomy & histology
Lupinus / genetics*
Lupinus / ultrastructure
Microscopy, Electron, Scanning
Phenotype
Polymorphism, Genetic
Seeds / anatomy & histology
Seeds / genetics*
Seeds / ultrastructure

Grants and funding

HR Smolice Sp. z o. o. Grupa IHAR provided support in the form of salaries for author Stanislaw Stawinski. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.