In order to fully explore the potential applications of nanoparticles in biopreservation, it is necessary to study the effect of nanoparticles on cell membrane permeabilities. The aim of this study is therefore to comparatively evaluate the osmotic responses of pig iliac endothelial cells in the absence and presence of commercially available hydroxyapatite nanoparticles. The results indicate that, after the introduction of 0.0 1 wt% hydroxyapatite nanoparticles, the dependence of cell membrane hydraulic conductivity (Lp) on temperature still obeys the Arrhenius relationship, while the reference value of the hydraulic conductivity of the cell membrane at 273.15K (Lpg) and the activation energy for water transport across cell membrane (ELp) change from 0.77 × 10(-14)m/Pa/s and 15.65 kJ/mol to 0.65 × 10(-14)m/Pa/s and 26.14 kJ/mol. That is to say, the reference value of the hydraulic conductivity of the cell membrane has been slightly decreased while the activation energy for water transport across cell membrane has been greatly enhanced, and thus it implies that the hydraulic conductivity of cell membrane are more sensitive to temperature in the presence of nanoparticles. These findings are of potential significance to the optimization of nanoparticles-aided cryopreservation.
Keywords: Activation energy; Hydraulic conductivity; Hydroxyapatite nanoparticles; Microperfusion chamber; Osmotically inactive cell volume.
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