Transcriptome profiles associated to VHSV infection or DNA vaccination in turbot (Scophthalmus maximus)

PLoS One. 2014 Aug 6;9(8):e104509. doi: 10.1371/journal.pone.0104509. eCollection 2014.

Abstract

DNA vaccines encoding the viral G glycoprotein show the most successful protection capability against fish rhabdoviruses. Nowadays, the molecular mechanisms underlying the protective response remain still poorly understood. With the aim of shedding light on the protection conferred by the DNA vaccines based in the G glycoprotein of viral haemorrhagic septicaemia virus (VHSV) in turbot (Scophthalmus maximus) we have used a specific microarray highly enriched in antiviral sequences to carry out the transcriptomic study associated to VHSV DNA vaccination/infection. The differential gene expression pattern in response to empty plasmid (pMCV1.4) and DNA vaccine (pMCV1.4-G860) intramuscular administration with regard to non-stimulated turbot was analyzed in head kidney at 8, 24 and 72 hours post-vaccination. Moreover, the effect of VHSV infection one month after immunization was also analyzed in vaccinated and non-vaccinated fish at the same time points. Genes implicated in the Toll-like receptor signalling pathway, IFN inducible/regulatory proteins, numerous sequences implicated in apoptosis and cytotoxic pathways, MHC class I antigens, as well as complement and coagulation cascades among others were analyzed in the different experimental groups. Fish receiving the pMCV1.4-G860 vaccine showed transcriptomic patterns very different to the ones observed in pMCV1.4-injected turbot after 72 h. On the other hand, VHSV challenge in vaccinated and non-vaccinated turbot induced a highly different response at the transcriptome level, indicating a very relevant role of the acquired immunity in vaccinated fish able to alter the typical innate immune response profile observed in non-vaccinated individuals. This exhaustive transcriptome study will serve as a complete overview for a better understanding of the crosstalk between the innate and adaptive immune response in fish after viral infection/vaccination. Moreover, it provides interesting clues about molecules with a potential use as vaccine adjuvants, antiviral treatments or markers for vaccine efficiency monitoring.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Fish Diseases* / metabolism
  • Fish Diseases* / prevention & control
  • Flatfishes
  • Novirhabdovirus*
  • Rhabdoviridae Infections* / metabolism
  • Rhabdoviridae Infections* / prevention & control
  • Rhabdoviridae Infections* / veterinary
  • Transcriptome / drug effects*
  • Vaccines, DNA / pharmacology*
  • Viral Vaccines / pharmacology*

Substances

  • Vaccines, DNA
  • Viral Vaccines

Associated data

  • GEO/GPL16776

Grants and funding

This work has been funded by the project CSD2007-00002 “Aquagenomics” and AGL2011-28921-C03 from the Spanish Ministerio de Ciencia e Innovación. The authors’ laboratory is also funded by the project 201230E057 from the Agencia Estatal Consejo Superior de Investigaciones Científicas (CSIC). P. Pereiro gratefully acknowledges the Ministerio de Educación for a FPU fellowship (AP2010-2408). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.