Abstract
α-1,4-Glucan lyases [glycoside hydrolase family (GH) 31] catalyze an elimination reaction to form 1,5-anhydro-d-fructose (AF), while GH31 α-glucosidases normally catalyze a hydrolytic reaction. We determined that a small amount of AF was produced by GH31 Aspergillus niger α-glucosidase from maltooligosaccharides by elimination reaction, likely via an oxocarbenium ion intermediate.
Keywords:
1,5-anhydro-d-fructose; GH31 α-glucosidase; elimination mechanism.
MeSH terms
-
Aspergillus niger / chemistry*
-
Aspergillus niger / enzymology
-
Biocatalysis
-
Carbohydrate Conformation
-
Carbon Isotopes
-
Fructose / analogs & derivatives*
-
Fructose / chemistry
-
Fungal Proteins / chemistry*
-
Fungal Proteins / isolation & purification
-
Hydrolysis
-
Kinetics
-
Oligosaccharides / chemistry*
-
alpha-Glucosidases / chemistry*
-
alpha-Glucosidases / isolation & purification
Substances
-
Carbon Isotopes
-
Fungal Proteins
-
Oligosaccharides
-
maltooligosaccharides
-
Fructose
-
1,5-anhydrofructose
-
alpha-Glucosidases