The interpretation of recent experimental evidence has lead to several discussions at international meetings which have included the suggestion that enamelins are not tissue-specific gene products but rather are similar to, or are identical to, albumin. Further complicating the issue has been the proposal by several investigators that albumin participates in the organization of hard tissues. The experimental strategy described in this study was to hybridize the mouse albumin gene cDNA clone, palb-3, to a Northern blot of secretory phase mouse mandibular first molar RNAs. This approach would permit the evaluation of the potential transcription of the albumin gene by differentiated ameloblasts as well as other cells of the odontogenic organ. The results of this approach indicated that odontogenic cells did not transcribe the albumin gene. Albumin polypeptides, therefore, cannot be synthesized by odontogenic cells and cannot be identical to odontogenic tissue-specific gene products. If albumin is incorporated into developing enamel matrix wherein it participates in undefined roles during enamel matrix organization, it cannot arise as a biosynthetic product of the odontogenic organ.