Transcription. Comment on "Mechanism of eukaryotic RNA polymerase III transcription termination"

Aneeshkumar G Arimbasseri; George A Kassavetis; Richard J Maraia

doi:10.1126/science.1253783

Transcription. Comment on "Mechanism of eukaryotic RNA polymerase III transcription termination"

Science. 2014 Aug 1;345(6196):524. doi: 10.1126/science.1253783. Epub 2014 Jul 31.

Authors

Aneeshkumar G Arimbasseri¹, George A Kassavetis², Richard J Maraia³

Affiliations

¹ Intramural Research Program of the Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
² Division of Biological Sciences, University of California, San Diego, CA, USA.
³ Intramural Research Program of the Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA. Commissioned Corps, U.S. Public Health Service. maraiar@mail.nih.gov.

Abstract

Nielsen et al. (Reports, 28 June 2013, p. 1577) characterized their RNA polymerase III (Pol III) preparation and concluded that it requires an RNA hairpin/duplex structure for terminating transcription. We could not corroborate their findings using bona fide Pol III from two laboratory sources. We show that Pol III efficiently terminates transcription in the absence of a hairpin/duplex in vitro and in vivo.

Publication types

Research Support, N.I.H., Intramural
Comment

MeSH terms

RNA Polymerase III / metabolism*
Saccharomyces cerevisiae / enzymology*
Transcription Termination, Genetic*

Substances

RNA Polymerase III

Abstract

Publication types

MeSH terms

Substances

Grants and funding