Inactivation effect of pressurized carbon dioxide on bacteriophage Qβ and ΦX174 as a novel disinfectant for water treatment

Huy Thanh Vo; Tsuyoshi Imai; Truc Thanh Ho; Masahiko Sekine; Ariyo Kanno; Takaya Higuchi; Koichi Yamamoto; Hidenori Yamamoto

doi:10.1016/S1001-0742(13)60603-8

Inactivation effect of pressurized carbon dioxide on bacteriophage Qβ and ΦX174 as a novel disinfectant for water treatment

J Environ Sci (China). 2014 Jun 1;26(6):1301-6. doi: 10.1016/S1001-0742(13)60603-8.

Authors

Huy Thanh Vo¹, Tsuyoshi Imai², Truc Thanh Ho³, Masahiko Sekine⁴, Ariyo Kanno⁴, Takaya Higuchi⁴, Koichi Yamamoto⁴, Hidenori Yamamoto⁴

Affiliations

¹ Faculty of Engineering, Yamaguchi University, 2-16-1 Tokiwadai, Yamaguchi 755-8611, Japan; Faculty of Urban Engineering, Central University of Construction, Tuy Hoa, Phu Yen, 56000, Vietnam. Electronic address: huypocrisy@gmail.com.
² Faculty of Engineering, Yamaguchi University, 2-16-1 Tokiwadai, Yamaguchi 755-8611, Japan. Electronic address: imai@yamaguchi-u.ac.jp.
³ Faculty of Urban Engineering, Central University of Construction, Tuy Hoa, Phu Yen, 56000, Vietnam.
⁴ Faculty of Engineering, Yamaguchi University, 2-16-1 Tokiwadai, Yamaguchi 755-8611, Japan.

PMID: 25079840
DOI: 10.1016/S1001-0742(13)60603-8

Abstract

The inactivation effects of pressurized CO2 against bacteriophage Qβ and ΦX174 were investigated under the pressure of 0.3-0.9 MPa, initial concentration of 10(7)-10(9) PFU/mL, and temperature of 17.8°C-27.2°C. The optimum conditions were found to be 0.7 MPa and an exposure time of 25 min. Under identical treatment conditions, a greater than 3.3-log reduction in bacteriophage Qβ was achieved by CO2, while a nearly 3.0 log reduction was observed for phage ΦX174. The viricidal effects of N2O (an inactivation gas with similar characteristics to CO2), normal acid (HCl), and CO2 treatment with phosphate buffered saline affirmed the chemical nature of CO2 treatment. The pumping cycle, depressurization rate, and release of intracellular substances caused by CO2 were its viricidal mechanisms. The results indicate that CO2 has the potential for use as a disinfectant without forming disinfection by-products.

Keywords: bacteriophage Qβ; bacteriophage ΦX174; microbubbles; pressurized CO(2); viricidal effect; water disinfection.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Allolevivirus*
Bacteriophage phi X 174*
Carbon Dioxide*
Disinfection*
Pressure
Virus Inactivation*
Water Purification*

Substances

Carbon Dioxide