The effect of chitosan and whey proteins-chitosan films on the growth of Penicillium expansum in apples

J Sci Food Agric. 2015 May;95(7):1475-81. doi: 10.1002/jsfa.6846. Epub 2014 Aug 26.

Abstract

Background: Penicillium expansum causes a major post-harvest disease of apples. The aim of this study was to investigate the inhibition effect of chitosan and whey proteins-chitosan films containing different amounts of quince and cranberry juice against P. expansum on the simulation medium and on apples. The mechanical properties of films were also evaluated.

Results: The presence of cranberry and quince juice in the composition of chitosan and whey proteins-chitosan films caused a significant (P ≤ 0.05) increase in elasticity and decrease in tensile strength of films. Chitosan and whey proteins-chitosan films with quince and cranberry juice demonstrated a significant (P ≤ 0.05) inhibition effect against P. expansum growth on the simulated medium and apples. The presence of cranberry juice in the composition of chitosan and whey proteins-chitosan films resulted in a longer lag phase and a lower P. expansum growth rate on the simulation medium in comparison with films made with the addition of quince juice. These differences were not evident when experiment was conducted with apples.

Conclusion: Addition of quince and cranberry juice to the chitosan and whey proteins-chitosan films as natural antifungal agents has some potential for prolonging the shelf life of apples.

Keywords: Penicillium expansum; apple; chitosan; edible film; whey proteins.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antifungal Agents / pharmacology
  • Chitosan / pharmacology*
  • Elasticity
  • Food Microbiology
  • Food Packaging / methods*
  • Food Preservation / methods
  • Fruit / microbiology*
  • Humans
  • Malus / microbiology*
  • Penicillium / drug effects*
  • Penicillium / growth & development
  • Plant Preparations / pharmacology*
  • Rosaceae
  • Tensile Strength
  • Vaccinium macrocarpon
  • Whey Proteins / pharmacology*

Substances

  • Antifungal Agents
  • Plant Preparations
  • Whey Proteins
  • Chitosan