Distribution of endocrine cells in the digestive tract of Alligator sinensis during the active and hibernating period

Huan Wang; Shengzhou Zhang; Naizhen Zhou; Chaolin Wang; Xiaobing Wu

doi:10.1016/j.tice.2014.06.005

Distribution of endocrine cells in the digestive tract of Alligator sinensis during the active and hibernating period

Tissue Cell. 2014 Oct;46(5):343-51. doi: 10.1016/j.tice.2014.06.005. Epub 2014 Jul 3.

Authors

Huan Wang¹, Shengzhou Zhang², Naizhen Zhou¹, Chaolin Wang³, Xiaobing Wu¹

Affiliations

¹ Key Laboratory for Conservation and Use of Important Biological Resources of Anhui Province, College of Life Sciences, Anhui Normal University, Wuhu, Anhui Province 241000, People's Republic of China.
² Key Laboratory for Conservation and Use of Important Biological Resources of Anhui Province, College of Life Sciences, Anhui Normal University, Wuhu, Anhui Province 241000, People's Republic of China. Electronic address: szzhang@mail.ahnu.edu.cn.
³ Alligator Research Center of Anhui Province, Xuanzhou 242000, People's Republic of China.

PMID: 25063208
DOI: 10.1016/j.tice.2014.06.005

Abstract

The digestive tract is the largest endocrine organ in the body; the distribution pattern of endocrine cells varies with different pathological and physiological states. The aim of the present study was to investigate the distributed density of 5-hydroxytryptamine (5-HT), gastrin (GAS), somatostatin (SS) and vasoactive intestinal peptide (VIP) immunoreactive (IR) cells in the digestive tract of Alligator sinensis during the active and hibernating period by immunohistochemical (IHC) method. The results indicated that 5-HT-IR cells were distributed throughout the entire digestive tract, which were most predominant in duodenum and jejunum. The density increased significantly in stomach and duodenum during hibernation. GAS-IR cells were limited in small stomach and small intestine. The density decreased significantly in small stomach during hibernation, while increased in duodenum. What's more, most of the endocrine cells in duodenum were generally spindle shaped with long cytoplasmic processes ending in the lumen during hibernation. SS-IR cells were limited in stomach and small stomach. The density increased in stomach while decreased in small stomach during hibernation, meanwhile, fewer IR cells occurred in small intestine. VIP-IR cells occurred in stomach and small stomach. The density decreased in small stomach, while increased in stomach during hibernation. These results indicated that the endocrine cells in different parts of digestive tract varied differently during hibernation, their changes were adaptive response to the hibernation.

Keywords: Alligator sinensis; Digestive tract; Endocrine cells; Hibernation; Immunohistochemical.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alligators and Crocodiles / anatomy & histology*
Alligators and Crocodiles / physiology*
Animals
Enteroendocrine Cells / cytology*
Hibernation*
Immunohistochemistry