Draft Genome Sequence of Marine Cyanobacterium Synechococcus sp. Strain NKBG042902, Which Harbors a Homogeneous Plasmid Available for Metabolic Engineering

Toru Honda; Yue Liang; Daichi Arai; Yasuhito Ito; Tomoko Yoshino; Tsuyoshi Tanaka

doi:10.1128/genomeA.00704-14

Draft Genome Sequence of Marine Cyanobacterium Synechococcus sp. Strain NKBG042902, Which Harbors a Homogeneous Plasmid Available for Metabolic Engineering

Genome Announc. 2014 Jul 24;2(4):e00704-14. doi: 10.1128/genomeA.00704-14.

Authors

Toru Honda¹, Yue Liang¹, Daichi Arai¹, Yasuhito Ito¹, Tomoko Yoshino, Tsuyoshi Tanaka²

Affiliations

¹ Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo, Japan.
² Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo, Japan tsuyo@cc.tuat.ac.jp.

Abstract

The marine cyanobacterium Synechococcus sp. strain NKBG042902 was isolated from coastal areas in Japan. Strain NKBG042902 has four plasmids: pSY8, pSY9, pSY10, and pSY11. Moreover, the hybrid plasmid pUSY02 containing pSY11 and Escherichia coli plasmid pUC18 was constructed for this strain. The genetic manipulation technique using pUSY02 was established for this strain and used in metabolic engineering. Here, we report the draft genome sequence of this strain, which has 77 contigs comprising a total length of 3,319,479 bp, with a G+C content of 49.4%.