Abstract
A polysaccharide fraction, HBE-III, was successfully purified in a high yield (40.4%) from its crude polysaccharide (HBE-0) which was prepared from pectinase hydrolysates of the peels of the Korean Citrus Hallabong. In experimental lung metastasis studies of Colon 26-M3.1 carcinoma cells, prophylactic administration of HBE-III significantly inhibited lung metastasis in a dose-dependent manner. In an in vitro cytotoxicity analysis, HBE-III (up to 1000 μg/mL) did not affect the growth of Colon 26-M3.1 cells and normal cells. HBE-III enhanced production of IL-6 and IL-12 by murine peritoneal macrophages. In an assay for natural killer (NK) cell activity, HBE-III (1000 μg/mouse, i.v.) significantly augmented NK cytotoxicity against Yac-1 tumor cells. The depletion of NK cells by injection of mouse anti-asialo GM1 serum abolished the inhibitory effect of HBE-III on lung metastasis of Colon 26-M3.1 cells. These data suggest that HBE-III may inhibit tumor metastasis via activation of macrophages and NK cells.
Keywords:
Antimetastasis activity; Citrus Hallabong peel; Natural killer cell; Polysaccharide.
Copyright © 2014 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antineoplastic Agents, Phytogenic / chemistry*
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Antineoplastic Agents, Phytogenic / isolation & purification
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Antineoplastic Agents, Phytogenic / therapeutic use*
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Cell Line
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Cell Line, Tumor
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Citrus / chemistry*
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Colon / immunology
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Colon / pathology
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Colonic Neoplasms / immunology
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Colonic Neoplasms / pathology*
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Female
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Hydrolysis
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Interleukin-12 / immunology
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Interleukin-6 / immunology
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Killer Cells, Natural / drug effects
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Killer Cells, Natural / immunology
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Killer Cells, Natural / pathology
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Lung / drug effects
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Lung / immunology
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Lung / pathology
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Lung Neoplasms / immunology
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Lung Neoplasms / pathology
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Lung Neoplasms / prevention & control*
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Lung Neoplasms / secondary*
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Mice
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Mice, Inbred BALB C
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Neoplasm Metastasis / immunology
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Neoplasm Metastasis / pathology
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Neoplasm Metastasis / prevention & control
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Pectins / chemistry*
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Pectins / isolation & purification
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Pectins / therapeutic use*
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Polysaccharides / chemistry
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Polysaccharides / isolation & purification
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Polysaccharides / therapeutic use
Substances
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Antineoplastic Agents, Phytogenic
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Interleukin-6
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Polysaccharides
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rhamnogalacturonan II
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Interleukin-12
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Pectins