Exploration of polar lipid accumulation profiles in Euglena gracilis using LipidBlast, an MS/MS spectral library constructed in silico

Takumi Ogawa; Takeshi Furuhashi; Atsushi Okazawa; Rai Nakai; Masami Nakazawa; Tobias Kind; Oliver Fiehn; Shigehiko Kanaya; Masanori Arita; Daisaku Ohta

doi:10.1080/09168451.2014.877826

Exploration of polar lipid accumulation profiles in Euglena gracilis using LipidBlast, an MS/MS spectral library constructed in silico

Biosci Biotechnol Biochem. 2014;78(1):14-8. doi: 10.1080/09168451.2014.877826. Epub 2014 Apr 10.

Authors

Takumi Ogawa¹, Takeshi Furuhashi, Atsushi Okazawa, Rai Nakai, Masami Nakazawa, Tobias Kind, Oliver Fiehn, Shigehiko Kanaya, Masanori Arita, Daisaku Ohta

Affiliation

¹ a Graduate School of Life and Environmental Sciences , Osaka Prefecture University , Sakai , Japan.

PMID: 25036478
DOI: 10.1080/09168451.2014.877826

Abstract

A rapid protocol for polar lipid profiling was applied to Euglena gracilis lipid metabolism by LipidBlast, an MS/MS spectral similarity search tool. The similarity search results suggested anoxia-induced polar lipid metabolism in Euglena characterized by the accumulation of differential lipid classes, carbon chain lengths, and unsaturated bond numbers. The informatics-supported MS spectral search provides an alternative option for global lipid profiling studies.

Keywords: Euglena gracilis; LC–MS/MS; LipidBlast; polar lipid profiling.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anaerobiosis
Computer Simulation*
Euglena gracilis / growth & development
Euglena gracilis / metabolism*
Lipid Metabolism*
Lipids / chemistry*
Tandem Mass Spectrometry / methods*

Substances

Lipids