Use of the BacT/alert system for rapid detection of microbial contamination in a pilot study using pancreatic islet cell products

Laura Murray; Neil McGowan; John Fleming; Laura Bailey

doi:10.1128/JCM.00447-14

Use of the BacT/alert system for rapid detection of microbial contamination in a pilot study using pancreatic islet cell products

J Clin Microbiol. 2014 Oct;52(10):3769-71. doi: 10.1128/JCM.00447-14. Epub 2014 Jul 16.

Authors

Laura Murray¹, Neil McGowan², John Fleming¹, Laura Bailey³

Affiliations

¹ Donation Testing Department, Scottish National Blood Transfusion Service, Glasgow, Scotland.
² SNBTS Cell Therapy Development Centre, Scottish National Blood Transfusion Service, The University of Edinburgh, Edinburgh bioQuarter, Edinburgh, Scotland.
³ SNBTS Cell Therapy Development Centre, Scottish National Blood Transfusion Service, The University of Edinburgh, Edinburgh bioQuarter, Edinburgh, Scotland Islet Isolation Laboratory, Tissues & Cells Directorate, Scottish National Blood Transfusion Service, Edinburgh, Scotland laurabailey2@nhs.net.

Abstract

At the Islet Isolation Laboratory of the Scottish National Blood Transfusion Service, manual sterility testing data show that contamination rates are 57.7% for pancreas transport fluid, 4.3% for postpurification islet samples, and 0% for pretransplant islet samples. This pilot study presents the BacT/Alert System as an alternative to manual testing to provide more rapid and sensitive sterility results for islet cell products.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Load / methods*
Biological Products*
Drug Contamination
Humans
Islets of Langerhans*
Pilot Projects
Time Factors

Substances

Biological Products