P22 mediated recombination of frt-sites

Angela Makumi; William Cenens; Rob Lavigne; Abram Aertsen

doi:10.1016/j.virol.2014.06.015

P22 mediated recombination of frt-sites

Virology. 2014 Aug:462-463:340-2. doi: 10.1016/j.virol.2014.06.015. Epub 2014 Jul 12.

Authors

Angela Makumi¹, William Cenens¹, Rob Lavigne², Abram Aertsen³

Affiliations

¹ Laboratory of Food Microbiology, Department of Microbial and Molecular Systems (M(2)S), Faculty of Bioscience Engineering, KU Leuven, Kasteelpark Arenberg 23, B-3001 Leuven, Belgium.
² Laboratory of Gene Technology, Department of Biosystems, Faculty of Bioscience Engineering, KU Leuven, Leuven, Belgium.
³ Laboratory of Food Microbiology, Department of Microbial and Molecular Systems (M(2)S), Faculty of Bioscience Engineering, KU Leuven, Kasteelpark Arenberg 23, B-3001 Leuven, Belgium. Electronic address: Abram.Aertsen@biw.kuleuven.be.

PMID: 25019493
DOI: 10.1016/j.virol.2014.06.015

Abstract

Flp mediated site specific recombination of frt-sites is frequently used in genetic engineering to excise, insert or invert DNA-cassettes in the chromosome. While constructs flanked by frt-sites are generally considered to be stable in the absence of the Flp enzyme, we observed that P22 chromosomes exceeding wild-type length tend to lose frt-flanked insertions via Flp independent recombination of frt-sites during phage propagation. This spontaneous recombination should be considered when engineering the chromosome of P22 and perhaps of other phages as well.

Keywords: Bacteriophage P22; Recombination; Salmonella Typhimurium; frt-sites.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteriophage P22 / genetics*
DNA, Viral / genetics*
Recombination, Genetic*

Substances

DNA, Viral