Abstract
The involvement of Ca(2+) in the response to high Mn(2+), Co(2+), Ni(2+), Cu(2+), Zn(2+), Cd(2+), and Hg(2+) was investigated in Saccharomyces cerevisiae. The yeast cells responded through a sharp increase in cytosolic Ca(2+) when exposed to Cd(2+), and to a lesser extent to Cu(2+), but not to Mn(2+), Co(2+), Ni(2+), Zn(2+), or Hg(2+). The response to high Cd(2+) depended mainly on external Ca(2+) (transported through the Cch1p/Mid1p channel) but also on vacuolar Ca(2+) (released into the cytosol through the Yvc1p channel). The adaptation to high Cd(2+) was influenced by perturbations in Ca(2+) homeostasis. Thus, the tolerance to Cd(2+) often correlated with sharp Cd(2+)-induced cytosolic Ca(2+) pulses, while the Cd(2+) sensitivity was accompanied by the incapacity to rapidly restore the low cytosolic Ca(2+).
Keywords:
Aequorin; Cadmium; Calcium signaling; Heavy metal stress; Saccharomyces cerevisiae.
Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Biological Transport / drug effects
-
Cadmium / metabolism
-
Cadmium / toxicity*
-
Calcium / metabolism
-
Calcium Signaling / drug effects*
-
Cation Transport Proteins / metabolism
-
Cell Membrane / drug effects
-
Cell Membrane / metabolism
-
Copper Transport Proteins
-
Cytosol / drug effects
-
Cytosol / metabolism
-
Dose-Response Relationship, Drug
-
Environmental Pollutants / metabolism
-
Environmental Pollutants / toxicity*
-
Homeostasis / drug effects
-
Iron-Binding Proteins / metabolism
-
Mutation
-
Oxidative Stress / drug effects
-
Saccharomyces cerevisiae / cytology*
-
Saccharomyces cerevisiae / drug effects*
-
Saccharomyces cerevisiae / genetics
-
Saccharomyces cerevisiae / metabolism
-
Saccharomyces cerevisiae Proteins / metabolism
-
Transcription Factors / metabolism
Substances
-
Cation Transport Proteins
-
Copper Transport Proteins
-
Environmental Pollutants
-
FET4 protein, S cerevisiae
-
Iron-Binding Proteins
-
Saccharomyces cerevisiae Proteins
-
Transcription Factors
-
YAP1 protein, S cerevisiae
-
Cadmium
-
Calcium