A surface-enhanced fluorescence (SEF) immunosensor for the detection of microcystin-LR was developed using Au nano-crosses as fluorescence enhancement nanoparticles and cy5 as a fluorescence label molecule. The SEF effects of cy5 in the proximity of Au nanorods and gold nano-crosses was investigated by using Au nanorods or nano-crosses coated negative-charged glass surfaces. Fluorescence measurements indicated that SEF was influenced by the size, shape and distribution of the Au nanoparticles, with an appropriate spacer layer between the Au nanoparticles and the cy5. The enhancement factor was from 2.3- to 35-fold. Under optimal conditions, the SEF immunosensor exhibited a good linear response at microcystin-LR concentrations of 0.02-16 ng mL(-1) (R(2)=0.9981). The limit of detection was 0.007 ng mL(-1) with little adsorption of microcystin-RR, microcystin-LW, and microcystin-LF. High microcystin-LR recoveries were obtained from naturally contaminated fish samples. The SEF immunosensor allows the reliable detection of microcystin-LR in seafood, and has potential in simple, sensitive detection applications.
Keywords: Au nano-crosses; Microcystin-LR; Surface enhanced fluorescence immunosensor.
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