The traditional polymerase chain reaction method was employed to obtain the complete mitochondrial genome of Microdevario kubotai. The mitogenome was determined to be a 16,566 bp long circular molecule. It contained the typical complement of 13 protein-coding genes, 2 rRNAs and 22 tRNAs. This is first report on the complete mitogenome sequence of M. kubotai.
Keywords: Microdevario kubotai; mitochondrial genome; polymerase chain reaction.