Preparation of polyphosphazene hydrogels for enzyme immobilization

Yue-Cheng Qian; Peng-Cheng Chen; Gui-Jin He; Xiao-Jun Huang; Zhi-Kang Xu

doi:10.3390/molecules19079850

Preparation of polyphosphazene hydrogels for enzyme immobilization

Molecules. 2014 Jul 8;19(7):9850-63. doi: 10.3390/molecules19079850.

Authors

Yue-Cheng Qian¹, Peng-Cheng Chen², Gui-Jin He³, Xiao-Jun Huang⁴, Zhi-Kang Xu⁵

Affiliations

¹ MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. education@zju.edu.cn.
² MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. chenpengcheng@zju.edu.cn.
³ MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. guikinghe@zju.edu.cn.
⁴ MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. hxjzxh@zju.edu.cn.
⁵ MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China. xuzk@zju.edu.cn.

Abstract

We report on the synthesis and application of a new hydrogel based on a methacrylate substituted polyphosphazene. Through ring-opening polymerization and nucleophilic substitution, poly[bis(methacrylate)phosphazene] (PBMAP) was successfully synthesized from hexachlorocyclotriphosphazene. By adding PBMAP to methacrylic acid solution and then treating with UV light, we could obtain a cross-linked polyphosphazene network, which showed an ultra-high absorbency for distilled water. Lipase from Candida rugosa was used as the model lipase for entrapment immobilization in the hydrogel. The influence of methacrylic acid concentration on immobilization efficiency was studied. Results showed that enzyme loading reached a maximum of 24.02 mg/g with an activity retention of 67.25% when the methacrylic acid concentration was 20% (w/w).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Enzyme Activation
Enzyme Stability
Enzymes, Immobilized*
Hydrogels / chemistry*
Lipase / chemistry
Methacrylates / chemistry
Organophosphorus Compounds / chemical synthesis
Organophosphorus Compounds / chemistry*
Polymers / chemical synthesis
Polymers / chemistry*
Thermodynamics

Substances

Enzymes, Immobilized
Hydrogels
Methacrylates
Organophosphorus Compounds
Polymers
poly(phosphazene)
methacrylic acid
Lipase