Abstract
The Na+-translocating NADH:ubiquinone oxidoreductase (Na+-NQR) from Vibrio cholerae is a membrane protein complex consisting of six different subunits NqrA-NqrF. The major domains of the NqrA and NqrC subunits were heterologously expressed in Escherichia coli and crystallized. The structure of NqrA1-377 was solved in space groups C222₁ and P2₁ by SAD phasing and molecular replacement at 1.9 and 2.1 Å resolution, respectively. NqrC devoid of the transmembrane helix was co-expressed with ApbE to insert the flavin mononucleotide group covalently attached to Thr225. The structure was determined by molecular replacement using apo-NqrC of Parabacteroides distasonis as search model at 1.8 Å resolution.
Keywords:
Na+-translocating NQR; Vibrio cholerae; covalently bound FMN.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / chemistry*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Cloning, Molecular
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Crystallization
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Crystallography, X-Ray
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Flavin Mononucleotide / chemistry*
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Gene Expression
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Membrane Proteins / chemistry*
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Protein Structure, Secondary
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Protein Structure, Tertiary
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Protein Subunits / chemistry*
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Protein Subunits / genetics
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Protein Subunits / metabolism
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Quinone Reductases / chemistry*
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Quinone Reductases / genetics
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Quinone Reductases / metabolism
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Structural Homology, Protein
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Vibrio cholerae / chemistry*
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Vibrio cholerae / enzymology
Substances
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Bacterial Proteins
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Membrane Proteins
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Protein Subunits
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Recombinant Proteins
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Flavin Mononucleotide
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sodium-translocating NADH-quinone reductase
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Quinone Reductases