DNA immunization of Mycobacterium tuberculosis resuscitation-promoting factor B elicits polyfunctional CD8(+) T cell responses

Clin Exp Vaccine Res. 2014 Jul;3(2):235-43. doi: 10.7774/cevr.2014.3.2.235. Epub 2014 Jun 20.

Abstract

Purpose: T cell-mediated immune responses, and particularly activation of polyfunctional T cells that simultaneously produce multiple cytokines, are necessary for the control of Mycobacterium tuberculosis. In the present study, we examined if DNA immunization of Mycobacterium tuberculosis resuscitation-promoting factor B (RpfB) elicits polyfunctional T cell responses in mice.

Materials and methods: C57BL/6 mice were immunized intramuscularly three times, at 3-week intervals, with RpfB-expressing plasmid DNA. For comparison, protein immunization was performed with recombinant RpfB in control mice. After immunization, RpfB-specific T cell responses were assessed by interferon-γ (IFN-γ) enzyme-linked immunosorbent spot assay and intracellular cytokine staining (ICS), and T cell polyfunctionality was assessed from the ICS data.

Results: RpfB DNA immunization induced not only humoral immune responses, but also CD8(+) and CD4(+) T cell responses. Immunodominant T-cell epitopes were identified within RpfB by assays with overlapping peptides. RpfB DNA immunization elicited a polyfunctional CD8(+) T cell response that was dominated by a functional phenotype of IFN-γ(+)/TNF-α(+)/IL-2(-)/CD107a(+).

Conclusion: RpfB DNA immunization elicits polyfunctional CD8(+) T cell responses, suggesting that RpfB DNA immunization might induce protective immunity against tuberculosis.

Keywords: DNA vaccines; Polyfunctional T cells; Resuscitation-promoting factor B; Tuberculosis.